Biography:
Zhang Ke-Qin was born in Qianxi, Guizhou in 1958. He graduated from Department of Plant Conservation, Guizhou Agricultural College in 1981. He received his master’s degree in plant pathology from Guizhou Agricultural College in 1989, and his Ph.D. in plant pathology from Chinese Agricultural University in 1998. He went to Reading University, UK, as a visiting scholar in 1989 and 1991, respectively. He became Vice-Dean of Department of Plant Conservation (1992), and Assistant to the President (1995) of Guizhou Agricultural College. He joined Yunnan University in 1998. Prof. Zhang now serves as Vice President of Yunnan University and Director of the Key Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education.Research Areas:
○ Resource collection and taxonomy of nematophagous microorganisms
○ Molecular mechanism of nematophagous micriorganis infect against nematodes
○ Natural immunity of nematodes
○ Metabolites of nematophagous microorganisms
○ Biological control of plant-parasitic nematodes Research Achievements:
● Collected and conserved systematically more than 8000 nematophagous microorganisms, and established the largest bank of microorganisms for nematode biocontrol in the world. ● Successfully developed the first microbial nematocide called XianchongbikeTM, in China. ● Revised and modified the systematic classification of nematophagous fungi based on extensive study of morphology, development, and molecular biology of these fungi. (Mycologia 2005, 97(5): 1034-1046) ● Firstly sequenced the whole genome of nematode-trapping fungus Arthrobotrys oligospora, and proposed a putative model for the trap formation of A. oligospora. (PLoS Pathogens 2011, 7: e1002179) ● Found one nematophagous bacterium Bacillus nematocida B16 has evolved a smart and effective strategy (“Trajan horse” mechanism) to kill nematodes. (PNAS 2010, 107: 16631-16636) ● Discovered a novel mechanism underlying killing of nematodes via mechanic force by two fungi, Coprinus comatus and Stropharia rugosoannulata. These two fungi effectively kill nematodes by producing spiny balls and acanthocytes, respectively. (AEM 2006, 72:2982-2987; AEM 2007, 73:3916 -3923 ) ● Resolved the tertiary structure of serine protease that is indispensable for the infection of nematodes. This enzyme has seven a-helix, 17 b-sheet and two disulfide bonds for the first time, and concluded that Ser99-Leu104 and Ser132-Gly136 residues of the enzyme play a critical role in recognition and orientation of its substrate, nematode cuticle. (FASEB J 2010, 24: 1391-1400) ● Isolated more than 36 new natural compounds that have nematocidal activities, Reported more than 65% new genes that are related to infection of nematodes from fungi and bacteria in the word. Selected Publications:
1. Jinkui Yang, Lei Wang, Xinglai Ji, Yun Feng, Xiaomin Li, Chenggang Zou, Jianping Xu, Yan Ren, Qili Mi, Junli Wu, Shuqun Liu, Yu Liu, Xiaowei Huang, Haiyan Wang, Xuemei Niu, Juan Li, Lianming Liang, Kaifang Ji, Wei Zhou, Zefen Yu, Guohong Li, Yajun Liu, Lei Li, Min Qiao, Lu Feng, Ke-Qin Zhang*. 2011. Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. PLoS Pathogen. DOI: 10.1371/journal.ppat.1002179.
2. Lianming Liang, Liu Shuqun, Yang Jinkui, Meng Zhaohui, Lei Liping, Ke-Qin Zhang*. 2011. Comparison of homology models and crystal structures of cuticle-degrading proteases from nematophagous fungi: structural basis of nematicidal activity. FASEB J 25(6): 1894-1902.
3. Qiuhong Niu, Ziaowei Huang, Lin Zhang, Jianping Xu, Dongmei Yang, Kangbi Wei, Xuemei Niu, Zhiqiang An, Joan Wennstrom Bennett, Chenggang Zou, Jinkui Yang, Ke-Qin Zhang*. 2010. A Trojan horse mechanism of bacterial pathogenesis against nematodes. Proc Natl Acad Sci USA 107, 16631-16636.
4. Gu W, Yang JK, Lou ZY, Liang LM, Sun YN, Huang JW, Li XM, Cao Y, Meng ZH, Ke-Qin Zhang*. 2011. Structural basis of enzymatic activity for the ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4. PloS One. 6(1): e16262
5. Lianming Liang, Zhaohui Meng, Fengping Ye, Jinkui Yang, Shuqun Liu, Yuna Sun, Yu Guo, Qili Mi, Xiaowei Huang, Chenggang Zou, Zihe Rao, Zhiyong Lou and Ke-Qin Zhang﹡. 2010. The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contrbution to infection against nematodes The FASEB Journal 24:1391-1400.
6. Chu, Y., Niu, X., Wang, Y., Guo, J., Pan, W., Huang, X., and Ke-Qin Zhang*. 2010. Isolation of Putative Biosynthetic Intermediates of Prenylated Indole Alkaloids from a Thermophilic Fungus Talaromyces thermophilus. Organic Letters 12: 4356-4359.
7. Juan Li, Li Yu, Jinkui Yang, Linqian Dong, Baoyu Tian, Zefen Yu, Lianming Liang, Ying Zhang, Xu Wang and Ke-Qin Zhang*. 2010. New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina. BMC Evolutionary Biology 10:68
8. Chenggang Zou, Huihui Tu, Xianyong Liu, Nan Tao, Wei Zhou, Ke-Qin Zhang*. 2010. PacC in the nematophagous fungus Clonostachys rosea controls virulence to nematodes. Environmental Microbiology. 12(7): 1868-1877.
9. Chenggang Zou, Nan Tao, Wenjing Liu, Jinkui Yang, Ziaowei Huang, Xianyong Liu, Huihui Tu, Zhongwei Gan and Ke-Qin Zhang*. 2010. Regulation of subtilisin-like protease prC expression by nematophagous fungus Clonostachy rosea. Environmental Microbiology. 12: 3243-52.
10. Chenggang Zou, Yongfang Xu, Wenjing Liu, Wei Zhou, Nan Tao, Huihui Tu, Xiaowei Huang, Jinkui Yang and Ke-Qin Zhang*. 2010. Expression of a Serine Protease Gene prC Is Up-Regulated by Oxidative Stress in the Fungus Clonostachy rosea: Implications for Fungal Survival. PLoS One 5(10):e13386.
11. JW Huang, JK Yang, YQ Duan, W Gu, XW Gong, Z Wei, C Su, Ke-Qin Zhang*. 2010. Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S rRNA sequence analysis. Appl Microbiol Biotechnol 88:553-562
12. Li GH, Dang LZ, Hong LJ, Zheng LJ, Liu FF, Li L, Liu YJ, Ke-Qin Zhang*. 2009. Nematicidal activity of honokiol and magnolol isolated from Magnolia tripetala. J Phytopathol 157: 390-392
13. Zhang Y, Yu ZF, Baral HO, Mo MH, Ke-Qin Zhang*. 2009. New species and records of Orbilia (Orbiliaceae, Ascomycetes) from China. Fungal Divers 36:141-153.
14. Zhang L, Yang J, Niu Q, Zhao X, Ye F, Liang L, Ke-Qin Zhang*. 2008. Investigation on the infection mechanism of the fungus Clonostachys rosea against nematodes using the green fluorescent protein. Appl Microbiol Biotechnol 78: 983-90
15. Tian BY, Yang JK, Lian LH, Wang CY , Ke-Qin Zhang*. 2007. Role of neutral protease from Brevibacillus laterosporus in pathogenesis of nematode. Appl Microbiol Biotechnol 74: 372-380.
16. Huang XW, Li YX, Niu QH, Ke-Qin Zhang*. 2007. Suppression Subtractive Hybridization(SSH) and its modifications in microbiological research. Appl Microbiol Biotechnol 76: 753-760.
17. Yang JK, Tian BY, Liang LM, Ke-Qin Zhang*. 2007. Extracellular enzymes and the pathogenesis of nematophagous fungi. Appl Microbiol Biotechnol 75:21-31.
18. Zhang Y, Yu ZF, Baral HO, Qiao M, Ke-Qin Zhang*. 2007. Pseudorbilia gen. nov.(Orbiliaceae) from Yunnan, China. Fung Divers 26: 1-7.
19. Luo H, Li X, Li GH, Ke-Qin Zhang*. 2006. Acanthocytes on Stropharia rugosoannulata function as a nematode-attacking device. Appl Environ Microbiol 72(4):2982-2987.
20. Yan Li, Kevin D. Hyde, Rajesh Jeewon, Lei Cai, Dhanasekaran Vijaykrishna, Ke-Qin Zhang*. 2005. Phylogenetics and evolution of Nematode-Trapping Fungi(Orbliales) estimated from nuclear and protein coding genes. Mycologia 97(5): 1034-1046.
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