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杨金奎简介

[发布时间]：2013-11-28 [点击次数]：

杨金奎 (Jinkui Yang)
1972年6月出生于云南省鹤庆县，博士，研究员。
Laboratory for Conservation and Utilization of Bio-resources,
Yunnan University
Kunming 650091
Yunnan, China
Tel.: 86 871-5032538:
Fax: 86 871 5034838;
E-mail: jinkui960@ynu.edu.cn
教育和工作经历
时间
教育和工作经历
1991年9月-1995年7月
山东大学微生物学系, 获理学学士
1995年8月-1998年8月
云南燃料二厂, 助理工程师, 微生物发酵
1998年9月-2001年7月
国家医药管理局四川抗菌素工业研究所, 微生物和生化药学，获硕士学位
2001年8月-2002年8月
云南瑞升科技有限公司, 助理工程师，烟草微生物
2002年9月-2005年6月
云南大学, 微生物学，获博士学位
2005年7月-
云南大学生物资源保护与利用重点实验室, 副研究员
Education ＆ Experience
Times
Education ＆ Experience
09/1991-07/1995
Study in Shandong University, Microbiology (B.S.)
08/1995-08/1998
Work in the No. 2 Fuel Factory, Assistant engineer
09/1998-07/2001
Study in Sichuan Industrial Institute of Antibiotics, Microbiology and Biochemical Pharmacy (M.D.)
08/2001-08/2002
Work in the Yunnan Reascend Tobacco Technology (Group) Co., LTD. Assistant engineer
09/2002-06/2005
Study in Yunnan University, Microbiology (PhD)
07/2005-present
Work in Laboratory for Conservation and Utilization of Bio-resources, and Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan University.

主要研究方向和兴趣：

1. 食线虫真菌侵染线虫的分子机理
1) 食线虫真菌侵染相关水解酶的克隆、表达和系统发育分析
2） 食线虫真菌侵染相关蛋白的晶体结构和功能研究
3） 少孢节丛孢的基因组测序和蛋白质组学研究
2. 烟草病原真菌抗药性分子机制研究 (e.g. 烟草赤星病，烟草黑胫病)
3. 微生物和酶在烟草加工中的应用
1） 烟碱的微生物分子代谢机制
2） 香兰素微生物合成途径关键酶的克隆、表达及晶体结构和功能研究
Research Interests：
1. Infection mechanism of nematophagous fungi against nematodes
1) Cloning, expression and phylogenetic analysis of infection-related enzymes from nematophagous fungi
2) Crystal structure and function analysis of infection-related proteins from nematophagous fungi
3) Sequencing of genome and proteomics analysis of Arthrobotrys oligospora
2. Drug resistance mechanism of tobacco pathogenic fungi (e.g. Alternaria longipes, Phytophthora parasitica)
3. Application of microorganisms and enzymes in tobacco processing
1) Molecular mechanism of nicotine degradation by microorganisms
2) Structure and function analysis of enzymes involved in biosynthesis of vanillin
承担的科研项目和成果：
在云南大学工作期间，主要从事食线虫真菌侵染线虫的分子机理和微生物及生化技术在烟草加工中的应用研究，逐步建立了从基因、蛋白、基因组和蛋白质组的研究平台。2006年以来主持国家自然科学基金4项（31272093, 31060012, 30960229 和30660107），云南省应用基础研究面上项目2项（2006C0004Q和2007C170M），2009年入选云南省中青年学术和技术带头人后备人才，2010年入选中国科学院西部之光人才培养计划。此外，作为骨干研究人员参与973项目、国家基金重点项目和云南省重点项目等多项科研课题的工作。
指导和协助指导研究生31名，24名研究生已经毕业，4名研究生获得云南省优秀硕士学位论文；指导16名本科生完成毕业论文研究。
获得2009年和2007年云南省科学技术奖（一等奖，排名3和6），获得2012年云南省科学技术奖（三等奖，排名3），获得2010年云南中烟工业有限责任公司科技进步一等奖（排名3），获得2010年中国烟草总公司云南省公司科技进步二等奖（排名8）。
2005年来，发表研究论文70篇，其中SCI论文53篇，中文核心刊物17篇。以第一作者或通讯作者在PLoS Pathog, Microbiology, Appl Microbiol Biotechnol和PLoS One等国际权威SCI杂志发表研究论文30篇，累计影响因子达到76.1。申请国家发明专利21项，已获得授权专利15项。
Publications (SCI, 2005 - present)
1) Yang JK, Wang L, Ji XL, Feng Y, Li XM, Zou CG, Xu JP, Ren Y, Mi QL, Wu JL, Liu SQ, Liu Y, Huang XW, Wang HY, Niu XM, Li J, Liang LM, Luo YL, Ji KF, Zhou W, Yu ZF, Li GH, Liu YJ, Li L, Qiao M, Feng L, Zhang KQ. 2011. Genomic and proteomic analyses of the fungus Arthrobotrys oligospora provide insights into nematode-trap formation. PLoS Pathog, 7: e1002179.
2) Gu W, Yang JK*, Lou ZY, Liang LM, Sun YN, Huang JW, Li XM, Cao Y, Meng ZH, Zhang KQ. 2011. Structural basis of enzymatic activity for the ferulic acid decarboxylase (FADase) from Enterobacter sp. Px6-4. PLoS One, 6(1): e16262 (*并列第一作者).
3) Su C, Lei LP, Duan YQ, Zhang KQ, Yang JK#. 2012. Culture-independent methods for studying environmental microorganisms: methods, application and perspective. Appl Microbiol Biotechnol, 93: 993-1003 (#通讯作者).
4) Gu Wen, Li Xuemei, Huang Jingwen, Yanqing Duan, Meng Zhaohui, Ke-Qin Zhang, Yang Jinkui#. 2011. Cloning, sequencing, and overexpression in Escherichia coli of the Enterobacter sp. Px6-4 gene for ferulic acid decarboxylase. Appl Microbiol Biotechnol, 89: 1797-1805.
5) Yang Jinkui, Xuna Zhao, Lianming Liang, Liping Lei, Zhenyuan Xia, Xuemei Niu, Chenggang Zou, Ke-Qin Zhang. 2011. Overexpression of a cuticle-degrading protease Ver112 increases the nematicidal activity of Paeciliomyces lilacinus. Appl Microbiol Biotechnol, 89: 1895-1903.
6) Su Can, Wen Gu, Wei Zhe, Ke-Qin Zhang, Yanqing Duan, Jinkui Yang#. 2011. Diversity and phylogeny of bacteria on Zimbabwe tobacco leaves estimated by 16S rRNA sequence analysis. Appl Microbiol Biotechnol, 92: 1033-1044.
7) Hongjuan Li, Xuemei Li, Yanqing Duan, Keqin Zhang, Jinkui Yang#. 2010. Biotransformation of nicotine by microbiology: the case of Pseudomans spp. Appl Microbiol Biotechnol, 86:11-17.
8) Huang Jingwen, Yang Jinkui*, Duan Yanqing, Gu Wen, Xiaowei Gong, Zhe Wei, Can Su, Zhang Keqin. 2010. Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S rRNA sequence analysis. Appl Microbiol Biotechnol, 88:553-562.
9) Yang JK, Gan ZW, Lou ZY, Tao N, Mi QL, Liang LM, Sun YN, Guo Y, Huang XW, Zou CG, Rao ZH, Meng ZH, Zhang KQ. 2010. Crystal structure and mutagenesis analysis of a chitinase CrChi1 from the nematophagous fungus Clonostachys rosea in complex with the inhibitor caffeine. Microbiology, 156: 3566-3574.
10) Qili Mi, Jinkui Yang*, Fengping Ye, Zhongwei Gan, Changwei Wu, Xuemei Niu, Chenggang Zou, Ke-Qin Zhang. 2010. Cloning and overexpression of Pochonia chlamydosporia chitinase gene pcchi44, a potential virulence factor in infection against nematodes. Process Biochem, 45：810-814.
11) Gong Xiaowei, Yang Jinkui*, Dong Jinyan, Zhe Wei, Li Qinghua, Wang Le, Duan Yanqin, Zhang Keqin. 2009. Isolation and characterization of Rhodococcus sp. Y22, and its potential application in tobacco processing. Res Microbiol, 160: 200-204.
12) Luo YY, Yang JK*, Zhu ML, Liu CJ, Li HY, Lu ZB, Pan WZ, Zhang ZH, Bi W, Zhang KQ. 2012. The group III two-component histidine kinase AlHK1 is involved in fungicides resistance, osmosensitivity, spore production and impacts negatively pathogenicity in Alternaria longipes. Curr Microbiol, 64: 449-556.
13) Yang JK, Lu ZB, Luo YY, Bi W, Zhu ML, Zhang KQ. 2012. Involvement of the putative G-protein α subunit gene pngpa1 in the regulation of growth, sensitivity to fungicides, and osmotic stress in Phytophthora nicotianae. Afri J Microbiol Res, 6: 680-689.
14) Gan ZW, Yang JK*, Tao N, Lou ZY, Mi Qili, Meng ZH, Zhang Ke-Qin. 2009. Crystallization and Preliminary Crystallographic Analysis of a Chitinase from Clonostachys rosea. Acta Cryst, F65, 386-388.
15) Liang LM, Liu SQ, Yang JK, Meng ZH, Lei LP, Zhang KQ. 2011. Comparison of homology models and crystal structures of cuticle-degrading proteases from nematophagous fungi: structural basis of nematicidal activity. FASEB J, 6：1894-1902.
16) Niu QH, Huang XW, Zhang L, Xu JP, Yang DM, Wei KB, Niu XM, An ZQ, Bennett JW, Zou CG, Yang JK, Zhang KQ. 2010. A Trojan horse mechanism of bacterial pathogenesis against nematodes. Proc Natl Acad Sci USA, 107: 16631-16636.
17) Zou CG, Tao N, Liu WJ, Yang JK, Huang XW, et al. 2010. Regulation of subtilisin-like protease prC expression by nematode cuticle in the nematophagous fungus Clonostachys rosea. Environ Microbiol, 12: 3243-52.
18) Juan Li, Li Yu, Jinkui Yang, Linqian Dong, Baoyu Tian, Zefen Yu, Lianming Liang, Ying Zhang, Xu Wang and Ke-Qin Zhang. 2010. New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina. BMC Evol Biol, 10: 68.
19) Jinkui Yang, Fengping Ye, Qili Mi, Songqing Tang, Juan Li, Keqin Zhang. 2008. Purification and cloning of an extracellular serine protease from the nematode-trapping fungus Monacrosporium cystosporium. J Microbiol Biotechnol, 18：852-858.
20) Jinkui Yang, Lianming Liang, Ying Zhang, Juan Li, Lin Zhang, Fengping Ye, Zhongwei Gan & Keqin Zhang. 2007. Purification and cloning of a novel serine protease from the nematophagous fungus Dactylellina varietas and its potential as a pathogenic factor against nematodes. Appl Microbiol Biotechnol, 75:557-565.
21) Jinkui Yang, Baoyu Tian, Lianming Liang & Keqin Zhang. 2007. Extracellular enzymes and the pathogenesis of nematophagous fungi. Appl Microbiol Biotechnol, 75:21-31.
22) Jinkui Yang, Juan Li, Lianming Liang & Keqin Zhang. 2007. Cloning and characterization of a cuticle-degrading protease from the nematode-trapping fungus Arthrobotrys conoides. Arch Microbiol, 188:167-174.
23) Zhang Lin, Yang Jinkui*, Qiuhong Niu, Xuna Zhao, Fengping Ye, Lianmin Liang, Ke-Qin Zhang. 2008. Investigation on the infection mechanism of the fungus Clonostachys rosea against nematodes using the green fluorescent protein. Appl Microbiol Biotechnol, 78:983-990.
24) Xuemei Li, Jinkui Yang*, Xian Li, Wen Gu, Jingwen Huang, Ke-Qin Zhang. 2008. Metabolism of ferulic acid via 4-vinylguaiacol to vanillin by a strain of Enterobacter sp. Px6-4 newly isolated from Vanilla root. Process Biochem, 43：1032-1037.
25) Zhongwei Gan, Jinkui Yang*, Nan Tao, Lianming Liang, Juan Li, and Keqin Zhang. 2007. Cloning of the gene Lecanicillium psalliotae chitinase Lpchi1 and identification of its potential role in the biocontrol of root-knot nematode Meloidogyne incognita. Appl Microbiol Biotechnol, 76:1309-1317.
欢迎对本组研究课题感兴趣的同学来实习和报考研究生，参与课题的研究！